Evaluation of Chemo-Preventive Effect of Methanolic Fruit Extract of Cucumis melo var. agrestis on DEN-Induced HCC in Sprague Dawley Rats

Abstract: Aim: To investigate chemo-preventive effect of methanolic fruit extract Cucumis melo var. agrestis on DEN (diethyl-nitrosamine) induced hepatocellular carcinoma in Sprague Dawley rats. Materials and Methods: Five groups rats randomly selected comprising 6 animals each group, all groups, except Group-1 were administered single dose (200 mg/kg, i.p. dissolved PBS) for the induction HCC promoted by phenobarbital (0.05%) 16 weeks. Group-I was only normal saline, however Group-3, 4 intervened standard (5-FU) Test-1 2 MECM. At end 16th week, overnight fasted sacrificed under anaesthesia then blood samples liver collected to analyse morphological liver, evaluate serum antioxidant, enzymatic, non-enzymatic, haematological parameters as well histopathology assess test (MECM) Results: As case treated low high mg/kg 400 mg/kg) MECM has showed mild moderate dependant anti-oxidant enzymatic non-enzymatic activity. However, other parameter such body weight, morphology, tumour incidence, results with respect drug very poor. Conclusion: This study demonstrated that clearly lack its ability act a agent, antioxidant might place it an effective adjuvant treatment HCC. Keywords: Diethyl-nitrosamine, Hepatocellular carcinoma, Methanolic agrestis, 5-Fluro-uracil Phosphate buffer solution.